The bioaccessibility of antioxidants also reached the highest values in the VI samples, in which the release of both the total phenols and total flavonoids to the liquid phase increased with in vitro digestion. Vacuum impregnated apple slices conferred maximum protection to the lactobacillus strain during its passage through the gastrointestinal tract, whereas drying with air reduced the final content of the living cells to values below 10 cfu/g. salivarius (CECT 4063) survival and the bioaccessibility of antioxidants during in vitro digestion. Accordingly, this study evaluates the impact of trehalose addition (10% w/w) and homogenization (100 MPa), together with the structural changes induced in vacuum impregnated apple slices (VI) by air-drying (AD) and freeze-drying (FD), on Lactobacillus salivarius spp. This involves both an effective release from the food matrix where they are contained and a high resistance to exposure to gastrointestinal conditions. To benefit the health of consumers, bioactive compounds must reach an adequate concentration at the end of the digestive process.
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